癫痫杂志

癫痫杂志

维生素 U 对丙戊酸钠所致大鼠肾毒性的保护作用

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目的 探讨维生素 U(Vit U) 对丙戊酸钠(VPA)所致大鼠肾毒性的保护作用,为临床应用提供实验室依据。 方法 将 48 只雌性 SD 大鼠随机分为 4 组(n=12),对照组(A 组)、VitU 组(B 组)、VPA 组(C 组)、Vit U+VPA 组(D 组)。A 组:等量生理盐水;B 组:Vit U 50 mg/(kg·d);C 组:VPA 300 mg/(kg·d);D 组:先给予 Vit U 50 mg/(kg·d),1 h 后 VPA 300 mg/(kg·d)。连续给药 2、4 周后,分别采血后开腹取肾(n=6)。检测血清总胆固醇 (TC)、甘油三酯 (TG)、高密度脂蛋白 (HDL)、低密度脂蛋白 (LDL)、肌酐(Cr)、尿素氮(BUN)及尿酸(UA)的水平。 结果 ① 血脂:2、4 周时与 A 组相比,C 组的 TC 及 LDL 均升高,差异具有统计学意义(P<0.05);② 肾功能血清生化指标:2 周时与 A 组相比较,B、C、D 三组的 Cr、BUN 及 UA,差异均无统计学意义(P>0.05);4 周时 C 组的 Cr、BUN 及 UA 水平与其他三组相比明显增高(P<0.05),差异具有统计学意义,D 组与 A 组相比较差异无统计学意义(P>0.05);③ 肾组织损伤:2 周时各组肾脏组织形态学无明显异常,4 周时仅 C 组可见炎性病灶,余各组无明显异常。 结论 VPA 可导致大鼠血脂水平升高,诱导的肾毒性可能与药物暴露时间有一定的关系;Vit U 对 VPA 所致大鼠的肾毒性具有一定的保护作用。

Objective The aim of present study was to investigate the protective effect of vitamin U on renal toxicity induced by sodium valproate (VPA) and provide laboratory data for clinical application of VPA. Methods In this study, 48 female rats were used. These animals were randomly divided into 4 groups: control group (group A), vitamin U group (group B), VPA group (group C), vitamin U+ VPA group (group D). Group A was given the same amount of normal saline, group B was given Vit U 50 mg/(kg·d), group C was given VPA 300 mg/(kg·d) and group D was given Vit U 50 mg/(kg·d) firstly, then VPA 300 mg/(kg·d) after 1 hours by gavage. After 2 or 4 weeks of continuous administration, the kidneys were collected from these rats after blood collection. Total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), serum creatinine (Cr), urea (BUN) and uric acid (UA) were detected by automatic biochemical analyzer. Result ① Blood lipid. There were significant differences in TC and LDL between the group A and group C (P<0.05), and the level of TC and LDL in group C were significantly higher. ② Serum biochemical indexes of renal function. There was no significant difference in Cr, UA and BUN four groups at 2w (P>0.05). At 4w, compared with the other three groups, the Cr, BUN and UA level of VPA group were significantly higher (P<0.05). But there was no significant difference between the group A and the group D. ③ Pathological morphology of renal tissue. At 2w, there was no obvious abnormality in renal structures among the four groups. At 4w, inflammatory lesions were only seen in VPA group, and mild inflammatory cell infiltration were seen in other three groups. Conclusion VPA can lead to a higher level of blood lipid. The renal toxicity induced by VPA may have a certain relationship with the time of drug exposure, and vitamin U has a protective effect on the renal toxicity induced by VPA.

关键词: 丙戊酸钠; 维生素 U; 肾功能; 大鼠

Key words: Valproic Acid; Vitamin U; Renal Function; Rat

引用本文: 江娇美, 陆晓妹, 张明, 赵晶, 冷宇, 吴鹏程, 王芬. 维生素 U 对丙戊酸钠所致大鼠肾毒性的保护作用. 癫痫杂志, 2018, 4(6): 486-491. doi: 10.7507/2096-0247.20180078 复制

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